Use of reverse transcription-PCR-based assays for quantification of HIV-1 in dried blood spots requires specific HIV-1 RNA isolation for monitoring of antiretroviral treatment efficiency.
نویسندگان
چکیده
François Rouet, Dramane Kania, Edouard Tuaillon, Almoustapha Maiga, Janin Nouhin, Christine Rouzioux, Diane Descamps, Jean-Christophe Plantier Unité VIH/Hépatites, Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Laboratoire de Virologie, Centre Muraz, Bobo-Dioulasso, Burkina Faso; INSERM U1058, Infection by HIV and by Agents with Mucocutaneous Tropism: from Pathogenesis to Prevention, Montpellier, France; Université Montpellier 1, Montpellier, France; Centre de Recherche et de Formation sur le VIH et la Tuberculose, Université des Sciences, des Techniques, et des Technologies de Bamako (USTTB), Bamako, Mali; Laboratoire de Virologie, CHU Necker Enfants Malades, Paris, France; Laboratoire de Virologie, IAME UMR 1137 INSERM, Université Paris-Diderot, Sorbonne Paris Cité, Groupe Hospitalier Bichat Claude-Bernard, Paris, France; Laboratoire Associé au Centre National de Référence du VIH, Rouen, France
منابع مشابه
Low-Cost HIV-1 Diagnosis and Quantification in Dried Blood Spots by Real Time PCR
BACKGROUND Rapid and cost-effective methods for HIV-1 diagnosis and viral load monitoring would greatly enhance the clinical management of HIV-1 infected adults and children in limited-resource settings. Recent recommendations to treat perinatally infected infants within the first year of life are feasible only if early diagnosis is routinely available. Dried blood spots (DBS) on filter paper a...
متن کاملSubtype variability, virological response and drug resistance assessed on dried blood spots collected from HIV patients on antiretroviral therapy in Angola.
BACKGROUND Subtype variability may influence treatment response and selection of drug resistance mutations in HIV-positive patients on antiretroviral therapy. PATIENTS AND METHODS A retrospective study was performed on specimens collected on dried blood spots (DBS) from HIV-positive individuals receiving antiretroviral therapy in Luanda, Angola. HIV-RNA, drug resistance mutations and subtypes...
متن کاملDrug- Resistance- Associated Mutations and HIV Sub-Type Determination in Drug-Naïve and HIV-Positive Patients under Treatment with Antiretroviral Drugs
Abstract Background and Objective: Resistance to antiretroviral agents is a significant concern in clinical management of HIV-infected individuals. Resistance is the result of mutations that develops in the viral protein targeted by antiretroviral agents. Material and Methods: In this cross-sectional study, the blood samples of 40 HIV-positive patients were collected. Twenty of them were d...
متن کاملHighly sensitive methods based on seminested real-time reverse transcription-PCR for quantitation of human immunodeficiency virus type 1 unspliced and multiply spliced RNA and proviral DNA.
The effectiveness of highly active antiretroviral therapy (HAART), the standard of care for the treatment of human immunodeficiency virus type 1 (HIV-1) infection, is assessed by measuring the viral RNA load in plasma. A patient is considered to be successfully treated when the HIV-1 load in plasma stays below the detection limit of commercial assays. However, virus replication and evolution do...
متن کاملMolecular assays for monitoring HIV infection and antiretroviral therapy.
Infection with HIV results in lifelong persistence of the virus in the body of infected persons, independent of antiretroviral treatment. Therefore, efficient and meaningful therapy monitoring has been developed since its introduction in the 1980s. Whereas, primarily, the measurement of the CD4 cell count was the most important clinical marker of disease progression, nowadays the estimation of ...
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 52 7 شماره
صفحات -
تاریخ انتشار 2014